Cell Freezing Cryopresevation Container

The Cell Freezing Container is a standard design in biological tissue culture, used to optimize the process of cell cryopreservation, providing a controlled cooling rate of -1°C/min, which is ideal for preserving cells. This system holds up to 18 tubes with various capacities (1mL, 1.2mL, 1.5mL, and 2mL) and includes a screw top lid to protect your samples in case of accidental tipping. The gradient cooling function, achieved with 100% isopropyl alcohol, helps maintain an optimal environment for cell freezing and resuscitation.

This tool is perfect for use in research and laboratories where preserving cell viability is critical.

Features:

• Capacity: Holds up to 18 tubes with 1mL, 1.2mL, 1.5mL, or 2mL capacity, saving valuable refrigeration space.
• Screw Cap: Prevents sample loss or contamination in case of accidental overturning.
• Reliable: Replaces unreliable foam containers and offers a more efficient solution.
• Easy to Use: Requires only 100% isopropyl alcohol and mechanical refrigeration for gradient cooling.
• Label Protection: Prevents contact with alcohol, preserving handwritten labels on test tubes.
• Temperature Resistance: Suitable for use in temperatures as low as -80°C.

Parameters:

• Material:
  • Polycarbonate body
  • Blue high-density polyethylene closure
  • White high-density polyethylene vial holder
  • Foam insert for insulation
• Closure Diameter: 120mm
• Box Diameter: 117mm
• Height: 86mm
• Suitable for Tubes: 1mL, 1.2mL, 1.5mL, and 2mL tubes
• Color: Clear body with a blue lid
• Application: Cell freezing and cell resuscitation
• Well Count: 18 slots

Package Includes:

• 1x Cell Freezing Container

This Cell Freezing Container is an essential tool for laboratories and research facilities that require controlled cooling for cell cryopreservation and resuscitation. It ensures safe and effective storage of biological samples, maintaining the integrity and viability of cells.

How to Use:

This method uses a programmed cooling box to gradually cool the samples from room temperature to -80°C, then store them in a liquid nitrogen tank for long-term preservation.

Protocol for Using the Program Cooling Box:

1. Step 1: Place the cell cryopreservation tubes into the cooling box stored at room temperature. Transfer the box into a -80°C environment, using isopropyl alcohol to achieve the gradient cooling. The next day, transfer the cells into a liquid nitrogen tank. Cells should be transferred promptly to avoid affecting the survival rate during resuscitation.
2. Step 2: The liquid level of isopropyl alcohol should always be above the scale mark on the external surface of the freezing box to ensure proper program cooling.
3. Step 3: Store the freezing box at room temperature. If the box has been taken from -80°C, allow it to warm to room temperature before use.
4. Step 4: Isopropanol should be replaced after about 5 uses. However, it is environmentally harmful, so it can be reused up to 6-7 times. Ensure the alcohol level is maintained at around 250ml; refill if necessary.
5. Step 5: DMSO in cryopreservation solutions can be toxic to cells, especially at room temperature. For best results, place the cryopreservation solution at 4°C before using it, and resuspend cells with the solution before freezing.

The programmed cooling box also plays a role in cell resuscitation by maintaining the gradient cooling function, which significantly improves cell survival rates during cryopreservation. The foam lining and isopropanol in the box help with both the freezing and the cell resuscitation process.